Dynamic change of paeoniflorin content in root of Paeonia lactiflora growing at Duolun County, Inner Mongolia / 中国中药杂志

<p><b>UNLABELLED</b>To explore the dynamic change of paeoniflorin content in root of Paeonia lactiflora.</p><p><b>METHOD</b>The samples were collected and paeoniflorin content was determined by HPLC.</p><p><b>RESULT</b>The results showed that the average content of paeoniflorin was the lowest at 3.05% in the roots collected in May, and during the fruit time it was rather high in August at 4.72%, and in September at 4.58%. The average content showed no significant differences during June to October.</p><p><b>CONCLUSION</b>Wild plant of P. lactiflora might be collected for medical use during blooming stage until wilting stage.</p>

Studies on the chemical constituents of Coeloglossum viride (L.) Hartm. var. bracteatum (Willd.) Richter / 药学学报

<p><b>AIM</b>To study the chemical constituents of the rhizomes of Coeloglossum viride (L.) Hartm. var. bracteatum (Willd.) Richter.</p><p><b>METHODS</b>The compounds were mainly isolated with normal phase and reverse phase column chromatographic methods and HPLC. Their structures were elucidated on the basis of physical constants and spectral analysis (UV, IR, EI-MS, positive and negative FAB-MS, APCI-MS, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, HMQC and HMBC).</p><p><b>RESULTS</b>Eight compounds were obtained from the ethanolic extract of the rhizomes of this plant, dactylorhin B (I), loroglossin (II), dactylorhin A (III), militarine (IV), coelovirin A (V), gastrodin (VI), thymidine (VII) and quercetin-3,7-di-O-beta-D-glucopyranoside (VIII).</p><p><b>CONCLUSION</b>All the compounds were obtained from this plant and genus Coeloglossum for the first time. V named coelovirin A, is a new compound.</p>

Studies on chemical constituents from Tibetan medicine wangla(rhizome of Coeloglossum viride var. bracteatum) / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Tibetan Madicine Wangla.</p><p><b>METHOD</b>Sepearing the chemical constituents by means of chromatography and identifying their structures on the basis of MS and NMR spectra and TLC with authentic samples.</p><p><b>RESULT</b>Eight compounds were isolated as 4-[(4-hydroxyphenyl) methoxy]benzenemethanol(I), 4,4'-dihydroxydiphenyl methane(II), 4,4'-dihydroxydibenzyl ether(III), gastrodin(IV), 4-hydroxy benzenemethanol(V), 4-hydroxybenz aldehyde(VI), beta-sitosterol(VII) and beta-daucosterol(VIII).</p><p><b>CONCLUSION</b>All the compounds were obtained from genus Coeloglassum for the first time. The compound I is a new natural product.</p>

Determination of flavone for Scutellaria baicalensis from different areas by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To describe the difference between native and nonative herbs by determining contents of seven kinds of flavone for twenty-five samples from seventeen areas.</p><p><b>METHODS</b>HPLC. Fluid phase: MEOH-H2O-CH3COOH(ICE) (41:59:0.2) and (50:50:0.2). Detection wavelength: 275.</p><p><b>RESULTS</b>The contents of baicalin are 6%-9%, wogenin are 2%-8%, baicalein are 0.1%-1.6%, neobaicalein are 0.01%-0.2%, wogonin are 0.01%-0.3%, visidulin and oroxylin are trace amounts or undetected.</p><p><b>CONCLUSION</b>The native and nonative herbs have no distinct differce in absolute component ratio. The ratio of baicalin and wogenin is under three. The ratio of baicalin and baicalein, baicalin and wogonin is between twenty and fifty.</p>

A study on genetic variation between wild and cultivated populations of Paeonia lactiflora Pall / 药学学报

<p><b>AIM</b>To discuss the genetic differentiation between wild and cultivated populations of Paeonia lactiflora Pall., and to find the reason for forming the genuineness of Radix Paeoniae rubra (Chishao) and Radix Paeoniae alba (Baishao).</p><p><b>METHODS</b>Forty three representative samples of P. lactiflora from 11 localities were analyzed by RAPD method with 21 random primers. According to RAPD results, the genetic diversity and genetic differentiation of P. lactiflora were detected by the percentage of polymorphic sites (PPB), the coefficient of gene differentiation (GST) and analysis of molecular variance (AMOVA).</p><p><b>RESULTS</b>(1) At species level, the PPB of P. lactiflora was 85.26%, the Nei's gene diversity (Ht) was 0.166. The PPB in wild population (WP) was 77.61%, which was more than that (54.96%) in cultivated population for medicine (MP), and that (61.76%) in cultivated population for ornament (OP). (2) AMOVA showed that 29.50% of the genetic diversity resulted from differentiation among populations. Pairwise Phist distance (0.3632) between WP and MP was furthest, while that (0.0973) between MP and OP was closest, indicating population differentiation was significant (P < 0.001). (3) In general, cluster analysis revealed that the samples of P. lactiflora were divided in wild and cultivated groups (except for 39). In WP, individuals of Duolun were separated from those of other localities. In MP, the clusters of samples corresponded well with their own habitats.</p><p><b>CONCLUSION</b>In addition to environmental factor, genetic differentiation should be the main cause for the genuineness of "Chishao" and "Baishao". Because of over collection and worse habitat, P. lactiflora in Duolun, whose root is the famous Chinese Geo-herbal--"Duolun Chishao", is progresively rare. So, it should become the endangered germplasm resource to protect.</p>

Study on the physicochemical properties of cultivated soil of genuine crude and no-enuine crude Chinese Angelica / 中国中药杂志

<p><b>OBJECTIVE</b>To provide basis for environmental factors of genuine crude Chinese angelica.</p><p><b>METHOD</b>On-the-spot investigation and indoor chemical analysis were made to study the physicochemical properties of cultivated soil of Chinese angelica.</p><p><b>RESULT AND CONCLUSION</b>The physical properties and the organic matte and mineral nutrition of cultivated soil of Chinese angelica are best in Min County, Gansu Province. The ecological environment is the leading factor in forming genuine crude Chinese angelica.</p>